Nutrient Agar 500gm Hi-Media, India

Intended use

Nutrient Agar is used as a general-purpose medium for the cultivation of less fastidious microorganisms and can be
enriched with blood or other biological fluids.

Composition**

• Peptone: 5.000 Gms / Litre
• Sodium chloride: 5.000 Gms / Litre
• HM peptone B#: 1.500 Gms / Litre
• Yeast extract: 1.500 Gms / Litre
• Agar: 15.000 Gms / Litre
• Final pH ( at 25°C): 7.4±0.2
• **Formula adjusted, standardized to suit performance parameters

Directions
Suspend 28.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. If desired, the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri plates.

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