Himedia Antibiotic Assay Medium (No.1), 500gm

Antibiotic Assay Medium No.1

Antibiotic Assay Medium No.1 is used for microbiological assay of ß-lactam and other antibiotics by the United
States Pharmacopoeia.

Ingredients (Gms / Litre)

• Peptone: 6.000
• Pancreatic digest of casein: 4.000
• Yeast extract: 3.000
• Beef extract: 1.500
• Dextrose: 1.000
• Agar: 15.000
• pH after sterilization: 6.6±0.1
  **Formula adjusted, standardized to suit performance parameters

Directions

Suspend 30.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Advice: Recommended for the microbiological assay of Bacitracin, Nafcillin, Cephalothin, Cephaperin, Cloxacillin,
Novobiocin, Penicillin-G

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