Himedia Lactobacillus MRS Broth, 500gm

Lactobacillus MRS Broth (MRS Broth)

Ingredients (Gms / Litre)

• Protease peptone: 10.000
• HM Peptone B# 10.000
• Yeast extract: 5.000
• Dextrose(Glucose) 20.000
• Polysorbate 80 (Tween 80) 1.000
• Ammonium citrate 2.000
• Sodium acetate 5.000
• Magnesium sulfate 0.100
• Manganese sulphate 0.050
• Dipotassium hydrogen phosphate 2.000
• Final pH ( at 25°C) 6.5±0.2

Directions
Suspend 55.15 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute in tubes, bottles, or flasks as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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